2,6-Dimethoxyphenol-Based Assay for Quantitation of Polysaccharide...
AbstractA rapid method for quantitation of lytic polysaccharide monooxygenase (LPMO) in multienzyme cocktails produced by mutant strains of filamentous fungi is developed. In this method,...
View ArticleCreation of Chitinase Producer and Disruption of Micromycete Cell Wall with...
AbstractA recombinant strain producing a complex of extracellular enzymes including chitinase from Myceliophtora thermophila was created based on the fungus Penicillium verruculosum. The activity of...
View ArticleExpression of CAS9 Nuclease of the CRISPR/Cas Genome Editing System in the...
AbstractThe cas9 gene of the Streptococcus pyogenes bacterium, which encodes Cas9 nuclease of the class 2 CRISPR/Cas system, is expressed in the filamentous fungus Penicillium verruculosum under the...
View ArticleCloning and Expression of a New Chitinase from Carnivorous Plants Drosera...
AbstractA novel chi19 gene encoding the chitinase, belonging to the glycosyl hydrolase family 19 from the predatory plant Drosera capensis is cloned and expressed in the E. coli bacterial system. The...
View ArticleExploring the Capabilities of the Penicillium verruculosum Expression System...
AbstractThis review summarizes the findings on the use of the Penicillium verruculosumВ1-537 (ΔniaD) recipient strain for the development of producers of enzymes—β-glucosidase, polysaccharide...
View ArticleConstruction of Recombinant Producers of Enzyme Preparations for Feed...
AbstractProducer strains have been created with an expression system based on the recipient strain Penicillium verruculosum 537 (ΔniaD) and the cellobiohydrolase-1 gene promoter. These strains made it...
View ArticleObtainment of a Complex Enzyme Preparation with Enhanced Pectinase Activity...
AbstractStudies were conducted to obtain a complex enzyme preparation that allows efficient hydrolysis of the main plant nonstarch polysaccharides (cellulose, xylans, and pectin). The goal of the...
View ArticleEnzymatic Destruction of Cellulose: Characteristics of the Kinetic...
AbstractThis paper studies the characteristics of the kinetic interaction of lytic polysaccharide monooxygenases (LPMOs) from Thielavia terrestris (TtLPMO), Penicillium verruculosum (PvLPMO), and...
View ArticleProduction of Industrial Enzymes Based on the Expression System of the Fungus...
AbstractThe review presents data on the creation of highly active recombinant producer strains of a wide range of enzymes for various fields of industry and agriculture with the expression system of...
View ArticleInfluence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the...
AbstractA method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The...
View ArticleA New Enzyme Preparation Containing Polysaccharide Monooxygenase and...
AbstractBased on the recipient strain of Penicillium verruculosum B1-537 (ΔniaD) using the promoter of the cellobiohydrolase I gene, a producer of homologous lytic polysaccharide monooxygenase (PMO)...
View ArticleCloning, Isolation, and Properties of a New Recombinant Tannase from the...
AbstractA new recombinant Aspergillus niger tannase (tannin acyl hydrolase) produced by the Penicillium verruculosum fungus has been studied. A strain with a high level of extracellular tannase (TAN2)...
View ArticleThe Synergistic Interaction of Arabinases of Different Types of Action in the...
AbstractThe interaction between endoarabinase (endoA) and exo-type enzymes has been studied during their joint hydrolysis of branched arabinane (BAra), sugar beet pulp (SBP), and apple pomace (AP). It...
View ArticleNew Feed Enzyme Preparations for The Destruction of Nonstarch Polysaccharides...
AbstractNew recombinant strains of Penicillium verruculosum with a high level of expression of homologous endo-β-1,4-glucanase II and heterologous phytase A of A. niger, as well as heterologous...
View ArticleCane Pretreatment by Deep Eutectic Solvents to Increase its Reactivity During...
AbstractCane was pretreated with a number of deep eutectic solvents (DESs) based on choline chloride (ChCl) as a hydrogen bond acceptor. Among hydrogen bond donors, lactic (LA) and oxalic (OA) acids...
View ArticleCarbohydrases: 50 Years of Research at the Department of Chemical Enzymology...
AbstractThis review describes the history of the development of research on carbohydrasеs conducted at the Department of Chemical Enzymology from the mid-1970s to the present time. The results...
View ArticleErratum to: Cane Pretreatment by Deep Eutectic Solvents to Increase its...
An Erratum to this paper has been published: https://doi.org/10.1134/S0003683823320030
View ArticleThe Preparation and Properties of Recombinant Forms of GH74 Family...
AbstractCloning and expression of the full-length endo-processive-type xyloglucanase from the Trichoderma reesei (TrXeg74A) fungus, as well as its catalytic domain TrXeg74A-CD, in the Penicillium...
View ArticleA Comparative Analysis of the Properties of Recombinant Endoinulinase,...
AbstractAs a result of cloning of the inuA, inu1, aglC, and fopA genes encoding endoinulinase (endoINU), exoinulinase (exoINU), α-galactosidase C (AGLС) and sucrase (SUC), respectively, into the...
View ArticleThe Function of the XlnR Transcription Factor in the Filamentous Fungus...
AbstractThe Penicillium verruculosum filamentous fungus is a highly active producer of cellulolytic complex enzymes, cellobiohydrolases, endoglucanases and β-glucosidases. Using the CRISPR/Cas9 genome...
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